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RT PCR Norovirus Detection Kit Lyophilized Rotavirus Test Fluorescence Probe

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RT PCR Norovirus Detection Kit Lyophilized Rotavirus Test Fluorescence Probe

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Brand Name : Biokey

Model Number : BIK-QL-H007 / BIK-QL-H007S

Certification : ISO 13485,CE

Place of Origin : Guangzhou China

MOQ : We can produce liquid and lyophilized kits

Price : USD

Payment Terms : L/C, T/T, Western Union

Supply Ability : 100,000 per day

Delivery Time : Depending on order quantity

Packaging Details : Carton package

Package : 96 T/kit (carton) / 48 T/kit (single tube)

Specimen : Faeces

Specimen preservation : 2 weeks ar 4-8℃,3 months at -20± 5℃ and for a long time below -70℃.

Applicable : Micgene 244/244 IVD(BIO-KEY)

Detection limit : 500 copies/mL

CV : ≤5%

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Multiplex RT-PCR four enteroviruses Fluorescence probing PCR test kit ( Lyophilized )


Intended use:

This kit is used for in vitro qualitative detection of Norovirus, Rotavirus, Adenovirus and Astrovirus nucleic acid RNA. It can be used for clinical auxiliary diagnosis of the etiological identification of suspected infected persons

Test Principle:


This kit uses fluorescent RT-PCR technology (TaqMan probe) to rapidly and accurately qualitatively detect the conserved regions of Norovirus/Rotavirus/Adenovirus/Astrovirus On the instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of Norovirus/Rotavirus/Adenovirus/Astrovirus through the change of fluorescent signal; the detection system contains a positive internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:

The product components as following:

1. BIK-QL-H007:

No. Components Amount
1 PCR Enzyme Mix (Lyophilized) 96 tests/bottle
2

Primer-Probe Mix 1 (Norovirus and

Rotavirus)

50 μL/vial
3

Primer-Probe Mix 2 (Adenovirus

and Astrovirus)

50 μL/vial
4 Enzyme Mix Buffer (5×) 400 μL/vial
5 Positive Control 100 μL/tube
6 Negative Control 100 μL/tube
7 RNase-free water 1mL/tube
8 Paraffin oil 1.5mL/tube*2

2. BIK-QL-H007S:

No. Components Amount
1 1 (Norovirus and Rotavirus Mix (Lyophilized)) 24 tests/kit
2 2 (Adenovirus and Astrovirus Mix (Lyophilized)) 24 tests/kit
3 Positive Control 100 μL/tube
4 Negative Control 100 μL/tube
5 RNase-free water 1mL/tube
6 Paraffin oil 1.5mL/tube

Data anlysis:

The following sample results are possible:

Ct value Result Analysis
1# No Ct Negative
2# ≤38 Positive
3# 38~40 Re-test; if it is still 38~40, report as 2#

Interpretation of test results:

(1)Norovirus and Rotavirus reaction:

FAM

(Norovirus)

VIC/HEX

(Rotavirus)

ROX

(IC)

Interpretation test results
1

-

-

-

Result invalid, test again
2 + - +/- Norovirus(+)
3 - + +/- Rotavirus(+)
4 + + +/- Norovirus and Rotavirus(+)
5 - - + Negative(-)

(2)Adenovirus and Astrovirus reaction:

FAM

(Adenovirus)

VIC/HEX

(Astrovirus)

ROX

(IC)

Interpretation test results
1

-

-

-

Result invalid, test again
2 + - +/- Adenovirus(+)
3 - + +/- Astrovirus(+)
4 + + +/- Adenovirus and Astrovirus(+)
5 - - + Negative(-)

NOTE: ‘+ ’ is positive, ‘- ’ is negative.

Operation details please check from IFU


Product Tags:

Norovirus Detection Kit Lyophilized

      

RT PCR Norovirus Detection Kit

      

Lyophilized Rotavirus Test Kit

      
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